王佳思
姓名：王佳思
邮箱：wangjs8@mail.sysu.edu.cn
主要经历：
2019.12-至今：中山大学，“百人计划”副教授，博士生导师
2016.3-2019.11：华盛顿大学，生物医学工程，博后 （合作导师：Daniel Chiu教授）
2010.8-2016.2：中科院长春应化所，化学生物学，博士 （导师：曲晓刚 教授）
2003.9-2010.7：吉林大学，生物化学与分子生物学，学士，硕士
研究方向：
课题组长期致力于开发新型生物分子检测技术与仪器，应用于重大疾病早期诊断、疾病现场快速检测，并致力于成果转化。具体研究方向包括：
1. 数字式分子诊断新技术。通过融合分子生物学、微流控芯片、分析化学等技术手段，研发新型数字单分子检测芯片与仪器，用于重大疾病的早期诊断。
2. 现场检测技术与仪器开发。通过结合微流控芯片、荧光纳米材料等技术，开发现场检测技术以及便携式仪器，实现疾病现场诊断。
科研项目：
1. 广东省自然科学基金面上项目
2. 科技部重点研发项目（参与）
3. 科技部重点研发青年项目（参与）
4. 科研院所与企业合作研发项目
成果总结：
以第一/通讯作者在国际著名学术期刊Science Advances, J Am Chem Soc, Advanced Science, Analytical Chemistry, Biosensors and Bioelectronics, ACS sensors等发表论文40余篇。文章引用次数超过4500次，H指数32。申请国外专利1项，国内专利8项。
代表性论文
1.Yingjie Cheng, Wenlong Guo, Yunzhu Wan, Sheng Li, Jianhua Zhou, Jiasi Wang* Ultrasensitive Detection of Nucleic Acid and Protein at Ambient Temperature by Using an Engineered CRISPR-Cas12a-Based Digital Assay. Anal Chem. 2025, 26;97(33):18355-18363. (中科院一区)
2.Zhina Wu, Rui Liu, Jianai Chen, Xueying Cai, Jingzheng Yi, Jiasi Wang*, Di Wang*, Min Hu*. An enzymatic cleavage-triggered minimally invasive nanosensor for urine-based detection of early atherosclerosis. Science Advances 2025, 11(11):eadu7614 (中科院一区)
3.Ke Wang, Haofan Yin, Sheng Li, Yunzhu Wan, Minmin Xiao, Xiaopeng Yuan, Zhenheng Huang, Yunxin Gao, Jianhua Zhou, Keying Guo, Jiasi Wang*. Quantitative detection of circular RNA and microRNA at point-of-care using droplet digital CRISPR/Cas13a platform. Biosens Bioelectron, 2025, 267, 116825. (中科院一区)
4.Yaqin Zhang, Jianai Chen, Zhina Wu, Chenfei Zhao, Rui Wang, Zhiping Li, Jiasi Wang*, Di Wang*. CRISPR/Cas Enzyme Catalysis in Liquid–Liquid Phase‐Separated Systems. Advanced Science, 2025, 12(3), 2407194. (中科院一区)
5.Yaqin Zhang, Yingjie Cheng, Chenfei Zhao, Jianai Chen, Zhina Wu, Jiasi Wang*, Di Wang*. Simultaneous detection of protein and nucleic acid biomarkers with a CRISPR-based assay. Chem Eng J, 2025, 503, 158452. (中科院一区)
6.Wenfei Xu, Xinyi Luo, Yunzhu Wan, Xiaonan Dong, Xuhui Li, Jiasi Wang*. Microfluidic-free droplet digital RAA/RT-RAA assisted CRISPR/Cas12a system for the detection of DNA/RNA viruses. Sensor Actuat B-Chem. 2025, 422, 136633. (中科院一区)
7.Xinyi Liao, Yunzhu Wan, Wenfei Xu, Sheng Li, Jianhua Zhou, Yanping Liu, Junhong Chen, Chongguang Yang, Jiasi Wang*, Xuhui Li*. Integrated detection of Mycobacterium tuberculosis at point-of-care using a streamlined digital CRISPR-based platform. Sensor Actuat B-Chem. 2025, 445, 138555. (中科院一区)
8.Sheng Li, Haofan Yin, Jiale Zheng, Yunzhu Wan, Ke Wang, Chongguang Yang, Jianhua Zhou, Meng Zhao, Xiaopeng Yuan, Jiasi Wang*. DECODE: contamination-free digital CRISPR platform for point-of-care detection of viral DNA/RNA. ACS Sensors, 2024, 9(8), 4256-4264. (中科院一区)
9.Yunzhu Wan, Sheng Li, Wenfei Xu, Ke Wang, Wenlong Guo, Chongguang Yang, Xuhui Li, Jianhua Zhou, Jiasi Wang*, Terminal chemical modifications of crRNAs enable improvement in the performance of CRISPR-Cas for point-of-care nucleic acid detection. Anal Chem. 2024, 96(41), 16346-16354. (中科院一区)
10.Xinyi Luo, Ke Wang, Yingying Xue, Xiaobao Cao, Jianhua Zhou, Jiasi Wang*, Digital PCR-free technologies for absolute quantitation of nucleic acids at single-molecule level, Chinese Chem Lett. 2024, 36(2), 109924. (中科院一区)
11.Yingying Xue，Xinyi Luo…Jiasi Wang*, PddCas: A Polydisperse Droplet Digital CRISPR/Cas-Based Assay for the Rapid and Ultrasensitive Amplification-Free Detection of Viral DNA/RNA. Anal Chem. 2023, 95(2):966-975. (中科院一区)
12.Ke Wang, Xinnan Bai, Yingying Xue, Xinyi Luo, Jianpei Dong, Gewei Yang, Kun Ma, Lu Huang, Jianhua Zhou, Jiasi Wang*, Absolute quantification of circRNA using digital reverse transcription-hyperbranched rolling circle amplification. Sensor Actuat B-Chem. 2023, 375, 132893. (中科院一区)
13.Xinyi Luo, Yunzhu Wan, Ke Wang, Qidong Wei, Ziming Yu, Lei Chen, Jianhua Zhou, Jiasi Wang*, Digital CRISPR/Cas12a-based platform for precise quantification of telomerase activity. Sensor Actuat B-Chem. 2023, 394, 134374. (中科院一区)
14.Wenfei Xu, Jie Peng, Chao Guo, Yingjie Chai, Haimeng Zhou, Jiasi Wang*, Xuhui Li*, Rapid and ultra-sensitive early detection of cervical cancer using CRISPR/Cas12-based assay based on methylated SEPT9, Sensor Actuat B-Chem. 2023, 379, 133231. (中科院一区)
15.Xinyi Luo, Yingying Xue, Enguo Ju, Yu Tao, Mingqiang Li, Li Zhou, Chongguang Yang, Jianhua Zhou, Jiasi Wang*. Digital CRISPR/Cas12b-based platform enabled absolute quantification of viral RNA. Anal Chim Acta. 2022, 1192, 339336 (JCR一区)
16.Yaqin Zhang, Minyan Chen, Chengrong Liu, Jiaqi Chen, Xinyi Luo, Yingying Xue, Qiming Liang, Li Zhou, Yu Tao, Mingqiang Li, Di Wang, Jianhua Zhou, Jiasi Wang*. Sensitive and rapid on-site detection of SARS-CoV-2 using a gold nanoparticle-based high-throughput platform coupled with CRISPR/Cas12-assisted RT-LAMP. Sensor Actuat B-Chem. 2021, 345, 130411. (中科院一区)
17.Jiasi Wang, Jeannette P. Staheli …, Daniel T. Chiu*. Detection of 14 high-risk human papillomaviruses using digital LAMP assays on a self-digitization chip. Anal Chem. 2021, 93(6):3266. (中科院一区)
18.Jiasi Wang, J Kreutz, A Thompson, Y Qin, A Sheen, J Wang, L Wu, S Xu, M Chang, D Raugi, R Smith, G Gottlieb and Daniel Chiu*. SD-chip enabled quantitative detection of HIV RNA using digital nucleic acid sequence-based amplification (dNASBA). Lab on Chip. 2018, 18(22):3501-3506. (JCR一区)
19.Jiasi Wang, Chuanqi Zhao, Jinsong Ren, Xiaogang Qu*. New Insights in Amyloid Beta Interactions with Human Telomerase. J Am Chem Soc. 2015, 137, 1213-9. (中科院一区)
20.Jiasi Wang, Li Wu, Jinsong Ren, Xiaogang Qu*. Visualizing Human Telomerase Activity With Primer Modified Au Nanoparticles. Small, 2012, 8, 259. (中科院一区)
发明专利：
(1) 王佳思，李晟，杨崇广，周建华; 一种基于液滴尺寸编码的多重数字核酸检测方法及应用, 2024-07-22, 中国，CN202410986349.0（发明专利）
(2) 王佳思，郭文龙，程莹洁，周建华; 一种基于CRISPR/Cas12酶的室温检测核酸或蛋白的方法，2024-07-05， 中国， 202410449463X（发明专利）
(3) 王佳思，李晟，周建华; 一种基于多分散液滴的数字核酸检测装置及其应用方法，2023-10-06，中国，202310826588.5（发明专利）
(4) 王佳思，万芸竹，罗心一, 周建华；一种基于修饰crRNA的CRISPR-Cas检测体系，2023-08-11，中国，202310547740.6 （发明专利）
(5) 王佳思，薛莹莹，王珂，周建华; 一种多分散液滴数字核酸检测方法及其应用, 2022-07-15, 中国, 202210482347.9 （发明专利）
(6) 王佳思，薛莹莹，白心楠; 一种多重单分子定量检测技术及其检测系统, 2021-11-05, 中国, 202111307579.2 （发明专利）
(7) 王佳思; 薛莹莹; 一种数字环介导等温扩增的方法, 2023-7-7, 中国, 202011235835.7（发明专利）
(8) CHIU, Daniel T.; YU, Jiangbo; KREUTZ, Jason E.; WANG, Jiasi; Digital Nucleic Acid Amplification Using Encoded Particle, 2018-1-11, WIPO, PCT/US2018/058803
招聘：
长期招聘生物医学工程、医学分子诊断、分析化学、医疗电子研究背景的副研究员和博士后，待遇优厚。
硕士研究生招生/本科实习：
欢迎保研/考研的本科同学加入实验室开展研究，课题组每名研究生均获得一等奖助金。欢迎各年级本科同学加入实验室实习并完成毕业设计，课题组研究生与本科生团队多次参加“全国生物医学工程创新设计大赛”并获得一/二等奖。推荐并鼓励本科同学发表研究论文并继续攻读学位或出国留学。
