A smartphone-assisted electrochemiluminescent biosensor for highly sensitive detection of miRNA-21 based on Ru(bpy)2(L)4+@MOF-5

  A smartphone-assisted electrochemiluminescent biosensor for highly sensitive detection of miRNA-21 based on Ru(bpy)2(L)4+@MOF-5

Zheng, K.; Zheng, Q. H., Mu, X. J.; Li, M. J.;* Yi, C. Q.*

Microchimica Acta, 2024

https://doi.org/10.1007/s00604-024-06675-9

Abstract. A smartphone-assisted electrochemiluminescence (ECL) strategy based on Ru(bpy)2(L)4+ as chromophores confined with metal − organic frameworks (Ru(bpy)2(L)4+@MOF-5) for the signal-amplified detection of miRNA-21 was developed. We synthesized a derivative of tris(2,2’-bipyridyl)ruthenium(II) complex (Ru(bpy)2(L)4+) with high charges, which can be loaded into the MOF-5 by strong electrostatic interaction to prevent from leakage. In addition, nucleic acid cycle amplification was used to quench the signal of Ru(bpy)2(L)4+@MOF-5 by ferrocene. This method was applied to detect the concentration of miRNA-21 ranging from 1.0 × 10−14-1.0 × 10−9 M with a low LOD of 7.2 fM. This work demonstrated the construction of a signal quenching strategy ECL biosensor for miRNA using Ru(bpy)2(L)4+@MOF-5 systems and its application in smartphone-assisted ECL detection. Graphical abstract: (Figure presented.) © The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2024.